Composite

Part:BBa_K1615034:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-01)


CBDcenA+Linker fused to MaoA driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in F1 origin.


Source

Monoamine Oxidase A from Klebsiella pneumoniae. CBDcenA from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington

References